phenotype microarray pm pm m1 pm m2  (Biolog Inc)

Journal: Nutrients

Article Title: Nutritional Niches of Cancer Therapy-Induced Senescent Cells

doi: 10.3390/nu14173636

Figure Lengend Snippet: Metabolic fingerprinting of therapy-induced senescence: an experimental approach. ( A ) A549 cells were cultured for 7 days with bleomycin (20 μmol/L), alisertib (0.5 μmol/L), doxorubicin (50 nmol/L), and palbociclib (5 μmol/L). Top: representative images of SA-β-gal staining from three independent experiments. Scale bar: 200 μm. Bottom: representative images from 6-well plates of 10-day clonogenic survival analyses of A549 cells previously cultured for 7 days with therapy-induced senescence agents. ( B ) Schematic representation of metabolite utilization analysis workflow in proliferative versus TIS cells using the Phenotype MicroArrays PM-M1 and PM-M2.

Article Snippet: In total, 50 μL/well of 400,000 cells/mL suspensions of TIS and proliferative cells (20,000 cells per well) in Biolog IF-M1 medium (RPMI-1640 medium lacking phenol red and depleted of carbon-energy sources, low glutamine [0.3 mmol/L], and low FBS [5%]), were transferred to Phenotype PM-M1 and PM-M2 MicroArrays (Biolog, Hayward, CA, USA) containing 190 biochemical substrates that could potentially be metabolized and provide energy for cells.

Techniques: Cell Culture, Staining

Journal: Nutrients

Article Title: Nutritional Niches of Cancer Therapy-Induced Senescent Cells

doi: 10.3390/nu14173636

Figure Lengend Snippet: Substrate utilization patterns of therapy-induced senescence cells. ( A ) Representative images of paired proliferative/TIS cells assayed in PM-M1 and PM-M2 plates. Negative control wells (blue boxes) have no substrate. Wells containing D-glucose (red boxes) served as positive controls. ( B ) Flower model Venn diagrams showing higher (left) or lower (right) substrate utilization in each type of TIS cell. ( C ) Analysis of mitochondrial oxidation of glucose and glutamine in proliferative and TIS cells using the Agilent Seahorse XF Mito Fuel Flex kit.

Article Snippet: In total, 50 μL/well of 400,000 cells/mL suspensions of TIS and proliferative cells (20,000 cells per well) in Biolog IF-M1 medium (RPMI-1640 medium lacking phenol red and depleted of carbon-energy sources, low glutamine [0.3 mmol/L], and low FBS [5%]), were transferred to Phenotype PM-M1 and PM-M2 MicroArrays (Biolog, Hayward, CA, USA) containing 190 biochemical substrates that could potentially be metabolized and provide energy for cells.

Techniques: Negative Control